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RT-PCR analysis of two laboratories demonstrated similar sensitivity of HPV detection in cervical tissue with the mixture of two primers

The detection of HPV type 16 by RT-PCR in archival specimens of cervical biopsies with the invasion of CIN I was found in 53.1% (9/17) of the patients. The PCR products were separated by agarose electrophoresis and visualized by ethidium bromide. Primers were selected in the early region of HPV-16 genome (E7, E1). The aim of the study was to evaluate reverse transcription-PCR (RT-PCR) in detection of variants of human papilloma virus (HPV) type 16 in patients with cervical intraepithelial neoplasia (CIN) I. Please email with ownership info and an address to where it was sent for verification.The application of reverse transcription-PCR in detection of variants of human papilloma virus type 16. This submission may be only be shared with the original sender and or the listed recipient. If you have any legal issues please contact the appropriate media file owner or post a link to where you found it online. Functional studies to determine the extent of burn depth damage demonstrated a relatively complete loss of back skin function in the superficial burn group but not at the deep level. The thermal damage pattern was similar to that at 10 days but the damage depths were greater due to re-epithelialization at 10 days. Histological examination at 10 days after injury demonstrated an average thermal damage zone of 3.4+/-0.2 mm at the superficial level, 17.0+/-1.0 mm at the intermediate level and 6.9+/-0.2 mm at the deep level. Thermal damage, as measured by thermocouples, followed a similar pattern. The temperature at the deep level rose to an average of 56 degrees C while at the intermediate level the temperature averaged 30 degrees C, and at the superficial level it averaged 26 degrees C. Histological examination at 4 days demonstrated an average thermal damage zone of 14.5+/-1.1 mm at the deep level and an average depth of penetration of 2.9+/-0.2 mm at this level. In vivo laser penetration was determined by measuring the temperature rise at various distances from the laser beam tip. At either 4 or 10 days after injury, a single laser pulse was applied just under the re-epithelialized margins to generate uniform thermal damage at three depths, a superficial, intermediate and deep level. Pig skin was burned acutely with a 4 watt neodymium-yttrium-aluminum-garnet (Nd-YAG) laser, and at 10 days the burn margins were re-epithelialized.

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